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KMID : 0613820170270040435
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Journal of Life Science
2017 Volume.27 No. 4 p.435 ~ p.441
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Analysis of Archaeal Community in Autotrophic Perchlorate-degrading Enrichment Culture
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Kim Young-Hwa
Do Sang-Hyun
So Hyun-Seung
Been Jun-Won
Sung Hae-Chan
Ji Sung-Chan
Son Myung-Hwa
Ahn Yeong-Hee
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Abstract
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Perchlorate (ClO4-) is an emerging contaminant detected in soil, groundwater, and surface water. Previous study revealed bacterial community in the enrichment culture tdegraded perchlorate using elemental sulfur as an electron donor. Quantitative and qualitative molecular methods were employed in this study to investigate archaeal community in the enrichment culture. Real-time qPCR showed that archaeal 16S rRNA gene copy number in the culture was about 1.5% of bacterial 16S rRNA gene copy number. This suggested that less archaea were adapted to the environment of the enrichment culture and bacteria were dominant. DGGE banding pattern revealed that archaeal community profile of the enrichment culture was different from that of the activated sludge used as an inoculum for the enrichment culture. The most dominant DGGE band of the enrichment culture was affiliated with Methanococci. Further research is necessary to investigate metabolic role of the dominant archaeal population to better understand microbial community in the perchlorate-reducing enrichment culture.
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KEYWORD
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Archaea, DGGE, enrichment culture, perchlorate, qPCR
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